...more research that further supports what we patients knew all along, that we were/are still sick with active infection beyond the Infectious Disease doctor recommended treatment period. It is not this never-proven hypothesis that we now had an auto-immune disease requiring no further antibiotics. These researchers showed this same thing in mice before but were criticized because miceas too removed from humans for it to mean anything. The rhesus macaque is as close to humans we are going to get without using humans.
Persistence of Borrelia burgdorferi in Rhesus Macaques following Antibiotic Treatment of Disseminated Infection."Our studies do however offer proof of the principle that intact spirochetes can persist in an incidental host comparable to humans, following antibiotic therapy. Additionally, our experiments uncover residual antigen associated with inflammatory foci."
Embers ME, Barthold SW, Borda JT, Bowers L, Doyle L, et al. (2012) Persistence of Borrelia burgdorferi in Rhesus Macaques following Antibiotic Treatment of Disseminated Infection. PLoS ONE 7(1): e29914. doi:10.1371/journal.pone.0029914
Abstract
The persistence of symptoms in Lyme disease patients following antibiotic therapy, and their causes, continue to be a matter of intense controversy. The studies presented here explore antibiotic efficacy using nonhuman primates.
Rhesus macaques were infected with B. burgdorferi and a portion received aggressive antibiotic therapy 4–6 months later.
Multiple methods were utilized for detection of residual organisms, including the feeding of lab-reared ticks on monkeys (xenodiagnosis), culture, immunofluorescence and PCR.
Antibody responses to the B. burgdorferi-specific C6 diagnostic peptide were measured longitudinally and declined in all treated animals.
B. burgdorferi antigen, DNA and RNA were detected in the tissues of treated animals.
Finally, small numbers of intact spirochetes were recovered by xenodiagnosis from treated monkeys.
These results demonstrate that B. burgdorferi can withstand antibiotic treatment, administered post-dissemination, in a primate host.
Though B. burgdorferi is not known to possess resistance mechanisms and is susceptible to the standard antibiotics (doxycycline, ceftriaxone) in vitro, it appears to become tolerant post-dissemination in the primate host.
This finding raises important questions about the pathogenicity of antibiotic-tolerant persisters and whether or not they can contribute to symptoms post-treatment.
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